As part of my fieldwork in China, I collected harvested tea leaves that were damaged by the tea green leafhopper. I want to quantify the amount of leafhopper damage for each harvest. I was able to find several solutions for quantifying holes in leaves or even damage to leaf margins, but typical leafhopper damage is just tiny brown spots on the undersides of leaves. I did find some tutorials on using ImageJ to analyze diseased area on leaves, but found that the leafhopper damage spots were too small and too similar in color to undamaged leaves for these tools to work reliably and be automated.
Eastern Beauty wulong tea is only produced from tea leaves damaged by leafhoppers. The induced volatiles produced by damaged tea plants gives the finished tea a unique flavor. How will leafhopper damage change in a warming climate, and how will that impact tea quality?
When plants experience mild or severe drought, how does that change their metabolic response to damage by insect herbivores?
How will elevated CO2 affect plant responses to insect herbivory? How will this interaction impact the quality of tea?